pGLO Lab Conclusion

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB N/A  we didn't do this  not part of lab - pGLO LB/amp 0 no colonies no colonies + pGLO LB/amp 12 milky white milky white + pGLO LB/amp/ara 16 milky white neon green

2.	What two new traits do your transformed bacteria have?
	The transformed bacteria have the ability to glow under UV light and resistance to the antibiotic, ampicillin.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	According to bio.net, bacteria with antibiotics divide every hour, and those without reproduce avery half hour. According to this method, that would result in 1048576 cells in a colony, multiply that by 16 and you get: 16777216
4.	What is the role of arabinose in the plates?
	Arabinose is the trigger that makes the growing fluorescent protein present.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	Scientists use GFP as a marker of proteins.They tag it to specific proteins in a cell and monitor the activity of it. It doesn't interfere with processes that occur within the organism but can be used to study the processes that occur. It is also used to track the spreading of a virus because the protein is inherited as the virus divides. It is also used commercially in pets like fishes to make them look interesting.
6.	Give an example of another application of genetic engineering. Genetic engineering is used in agriculture to create crops with more desirable traits. An example of this would be corn. It is modified to be resistant to a pesticide called herbicide glyphosate which is a weed killer.